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Molecular phylogeny of the genus Dactylopius (Hemiptera: Dactylopiidae) and identification of the symbiotic bacteria.

Authors
  • Ramírez-Puebla, S T
  • Rosenblueth, M
  • Chávez-Moreno, C K
  • de Lyra, M C Catanho Pereira
  • Tecante, A
  • Martínez-Romero, E
Type
Published Article
Journal
Environmental Entomology
Publisher
Oxford University Press
Publication Date
Aug 01, 2010
Volume
39
Issue
4
Pages
1178–1183
Identifiers
DOI: 10.1603/EN10037
PMID: 22127169
Source
Medline
License
Unknown

Abstract

Phylogenetic analyses, from polymerase chain reaction (PCR)-amplified 12S rRNA and 18S rRNA gene sequences from cochineal insects of the genus Dactylopius present in Mexico, showed that D. ceylonicus, D. confusus, and D. opuntiae are closely related. D. coccus constitutes a separate clade, and D. tomentosus is the most distantly related. Bacterial 16S rRNA sequences from all the Dactylopius species sampled showed a common β-proteobacteria, related to Azoarcus, also found in eggs and in bacteriocytes in D. coccus. We propose the name "Candidatus Dactylopiibacterium carminicum" for this endosymbiont. Other bacterial sequences recovered from the samples were close to those from soil or plant associated bacteria, like Massilia, Herbaspirillum, Acinetobacter, Mesorhizobium, and Sphingomonas, suggesting a possible horizontal transmission from Cactaceae plant sap to Dactylopius spp. during feeding. This is the first molecular analysis of Dactylopius species and of their associated bacteria.

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