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Molecular Mechanisms of Proline-Mediated Tolerance to Toxic Heavy Metals in Transgenic Microalgae

Authors
Publisher
American Society of Plant Biologists
Publication Date
Source
PMC
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  • Biology
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Abstract

Molecular Mechanisms of Proline-Mediated Tolerance to Toxic Heavy Metals in Transgenic Microalgae by Surasak Siripornadulsil, Samuel Traina, Desh Pal S. Verma, and Richard T. Sayre Plantcell Volume 14(11):2837-2847 November 1, 2002 ©2002 by American Society of Plant Biologists Growth Rates and Chlorophyll Contents of Wild-Type and P5CS-Expressing Transgenic Algae Grown in the Presence and Absence of Cd.(A) Growth rate in the absence of Cd.(B) Growth rate in the presence of 100 μM Cd.(C) Chlorophyll content in the absence of Cd.(D... Siripornadulsil S et al. Plantcell 2002;14:2837-2847 ©2002 by American Society of Plant Biologists Survival of Wild-Type and P5CS-1 Transgenic Algae in Seawater.Cells were incubated with artificial seawater for 1, 3, 5, 12, or 24 h before growing in Tris-acetate-phosphate medium supplemented with Arg. Siripornadulsil S et al. Plantcell 2002;14:2837-2847 ©2002 by American Society of Plant Biologists Expression of the Mothbean P5CS Protein and Pro Production in Transgenic Algae.(A) Protein gel blot of total soluble proteins from P5CS transgenic algae (lanes 1 to 3) and wild-type (W.T.) algae (lanes 4 to 6). Siripornadulsil S et al. Plantcell 2002;14:2837-2847 ©2002 by American Society of Plant Biologists Cd-K Edge X-Ray Absorption Spectra (A), EXAFS Spectra (B), and EXAFS Fourier Transform (C) of Cd-GSH, Cd-Pro, and Cd-Chlamydomonas Complexes.Waves are as follows: A, GSH-Cd(NO3)2 solution; B, Pro-Cd(NO3)2 solution; C, GSH-Pro-Cd(NO3)2 solution; D, Chlamydom... Siripornadulsil S et al. Plantcell 2002;14:2837-2847 ©2002 by American Society of Plant Biologists MDA Content and GSH:0.5 GSSG Redox State of Wild-Type (black bars) and P5CS-1–Expressing Transgenic Algae (gray bars).(A) MDA content per cell.(B) Redox state of cells as measured by GSH content.(C) GSSG content per cell.(D) GSH:0.5 GSSG redox ratio.Data ar... Siripornadulsil S et al. Plantcell 2002;14:2837-2847 ©2002 by American Society of Plant Biologists Role of Free Pro in Free Radical Quenching.Ph

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