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Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides.

Authors
  • Tamura, Kazune1
  • Hemsworth, Glyn R2
  • Déjean, Guillaume3
  • Rogers, Theresa E4
  • Pudlo, Nicholas A4
  • Urs, Karthik4
  • Jain, Namrata5
  • Davies, Gideon J2
  • Martens, Eric C4
  • Brumer, Harry6
  • 1 Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4, Canada; Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada. , (Canada)
  • 2 York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK.
  • 3 Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4, Canada. , (Canada)
  • 4 Department of Microbiology and Immunology, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, MI 48109, USA.
  • 5 Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4, Canada; Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada. , (Canada)
  • 6 Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4, Canada; Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada; Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada; Department of Botany, University of British Columbia, 3200 University Boulevard, Vancouver, BC V6T 1Z4, Canada. Electronic address: [email protected] , (Canada)
Type
Published Article
Journal
Cell Reports
Publisher
Elsevier
Publication Date
Oct 10, 2017
Volume
21
Issue
2
Pages
417–430
Identifiers
DOI: 10.1016/j.celrep.2017.09.049
PMID: 29020628
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Microbial utilization of complex polysaccharides is a major driving force in shaping the composition of the human gut microbiota. There is a growing appreciation that finely tuned polysaccharide utilization loci enable ubiquitous gut Bacteroidetes to thrive on the plethora of complex polysaccharides that constitute "dietary fiber." Mixed-linkage β(1,3)/β(1,4)-glucans (MLGs) are a key family of plant cell wall polysaccharides with recognized health benefits but whose mechanism of utilization has remained unclear. Here, we provide molecular insight into the function of an archetypal MLG utilization locus (MLGUL) through a combination of biochemistry, enzymology, structural biology, and microbiology. Comparative genomics coupled with growth studies demonstrated further that syntenic MLGULs serve as genetic markers for MLG catabolism across commensal gut bacteria. In turn, we surveyed human gut metagenomes to reveal that MLGULs are ubiquitous in human populations globally, which underscores the importance of gut microbial metabolism of MLG as a common cereal polysaccharide. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

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