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Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals

Authors
  • Goeman, F.
  • Manni, I.
  • Artuso, S.
  • Ramachandran, B.
  • Gabriele Toietta
  • Bossi, G.
  • Rando, G.
  • Cencioni, C.
  • Germoni, S.
  • Straino, S.
  • Capogrossi, M. C.
  • Bacchetti, S.
  • Maggi, A.
  • Sacchi, A.
  • Ciana, P.
  • Piaggio, G.
Type
Published Article
Journal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology
Publication Date
Feb 26, 2012
Volume
23
Issue
8
Pages
1467–1474
Identifiers
DOI: 10.1091/mbc.e12-01-0039
PMID: 22379106
PMCID: PMC3327325
Source
MyScienceWork
Keywords
Funders
  • Associazione Italiana per la Ricerca sul Cancro
  • Fondazione Cariplo
  • Associazione Italiana per la Ricerca sul Cancro
  • Fondazione Cariplo
License
Green

Abstract

In vivo imaging involving the use of genetically engineered animals is an innovative powerful tool for the noninvasive assessment of the molecular and cellular events that are often targets of therapy. On the basis of the knowledge that the activity of the nuclear factorY (NF-Y) transcription factor is restricted in vitro to proliferating cells, we have generated a trans-genic reporter mouse, called MITO-Luc (for mitosis-luciferase), in which an NF-Y-dependent promoter controls luciferase expression. In these mice, bioluminescence imaging of NF-Y activity visualizes areas of physiological cell proliferation and regeneration during response to injury. Using this tool, we highlight for the first time a role of NF-Y activity on hepatocyte proliferation during liver regeneration. MITO-Luc reporter mice should facilitate investigations into the involvement of genes in cell proliferation and provide a useful model for studying aberrant proliferation in disease pathogenesis. They should be also useful in the development of new anti/ proproliferative drugs and assessment of their efficacy and side effects on nontarget tissues.

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