Molecular identification of tick-borne hemoparasites in equines from Northwestern Colombia

Affordable Access

Download Read

Molecular identification of tick-borne hemoparasites in equines from Northwestern Colombia

Authors
  • Revista MVZ Córdoba
  • Yeison Agudelo-Ruíz1
  • Leidy Acevedo-Gutiérrez1
  • Andrés Montoya-Sanchéz1
  • Luis Paternina T2
  • Juan Rodas G1
Type
Published Article
Journal
Revista Mvz Córdoba
Publisher
Universidad de Cordoba
Publication Date
May 22, 2017
Accepted Date
Jan 23, 2017
Volume
22
Issue
supl
Pages
6004–6013
Identifiers
DOI: 10.21897/rmvz.1070
Source
MyScienceWork
Keywords
License
Green

Abstract

Objetive. To detect and identify Anaplasmataceae agents and piroplasms in equines from the slaughterhouse "La Rinconada" at Rionegro municipality in Antioquia. Materials and Methods. A descriptive cross-sectional study was carried out on equines selected by convenience during a period of 2015. Information about species, sex, age and origin of the animals. Whole blood was collected for DNA extraction procedure, and a PCR targeting a 360bp of Anaplasmataceae 16S ribosomal gene and 450bp of 18S ribosomal gene of Piroplasm were used for detection. PCR amplicons selected were submitted to direct sequencing for identification of hemoparasites through genetic analysis. Results. 135 equine samples from Antioquia, Cordoba y Sucre were analyzed. 78% were horses, 16% were donkeys and 6% were mules. Anaplasmataceae agents were not detected in any sample, meanwhile 13% were positive to piroplasm PCR. Sequence analysis reveals the circulation of Theileria equi in northwestern Colombia. Conclusion. This work presents the first molecular evidence of at least three genotypes of T. equi in equines of northwestern Colombia.

Report this publication

Statistics

Seen <100 times
Downloaded <100 times