Affordable Access

Access to the full text

Molecular Fitness Landscapes from High-Coverage Sequence Profiling

Authors
  • Blanco, Celia
  • Janzen, Evan
  • Pressman, Abe
  • Saha, Ranajay
  • Chen, Irene A.
Type
Published Article
Journal
Annual Review of Biophysics
Publisher
Annual Reviews
Publication Date
May 06, 2019
Volume
48
Pages
1–18
Identifiers
DOI: 10.1146/annurev-biophys-052118-115333
Source
Annual Reviews
Keywords
License
Yellow

Abstract

The function of fitness (or molecular activity) in the space of all possible sequences is known as the fitness landscape. Evolution is a random walk on the fitness landscape, with a bias toward climbing hills. Mapping the topography of real fitness landscapes is fundamental to understanding evolution, but previous efforts were hampered by the difficulty of obtaining large, quantitative data sets. The accessibility of high-throughput sequencing (HTS) has transformed this study, enabling large-scale enumeration of fitness for many mutants and even complete sequence spaces in some cases. We review the progress of high-throughput studies in mapping molecular fitness landscapes, both in vitro and in vivo, as well as opportunities for future research. Such studies are rapidly growing in number. HTS is expected to have a profound effect on the understanding of real molecular fitness landscapes.

Report this publication

Statistics

Seen <100 times