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Molecular detection of Gram-positive bacteria in the human lung through an optical fiber-based endoscope.

Authors
  • Mills, Bethany1
  • Megia-Fernandez, Alicia2
  • Norberg, Dominic3
  • Duncan, Sheelagh3
  • Marshall, Adam3
  • Akram, Ahsan R3
  • Quinn, Thomas3
  • Young, Irene3
  • Bruce, Annya M3
  • Scholefield, Emma3
  • Williams, Gareth O S3
  • Krstajić, Nikola3
  • Choudhary, Tushar R3, 4
  • Parker, Helen E3, 5
  • Tanner, Michael G3, 6
  • Harrington, Kerrianne7
  • Wood, Harry A C7
  • Birks, Timothy A7
  • Knight, Jonathan C7
  • Haslett, Christopher3
  • And 4 more
  • 1 Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK. [email protected] , (France)
  • 2 School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh, EH9 3FJ, UK.
  • 3 Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK. , (France)
  • 4 The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK.
  • 5 Department of Applied Physics, Royal Institute of Technology, KTH, SE-106 91, Stockholm, Sweden. , (Sweden)
  • 6 Scottish Universities Physics Alliance (SUPA), Institute of Photonics and Quantum Sciences, Heriot-Watt University, Edinburgh, EH14 4AS, UK.
  • 7 Centre for Photonics and Photonic Materials, Department of Physics, University of Bath, Bath, BA2 7AY, UK.
  • 8 School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh, EH9 3FJ, UK. [email protected]
  • 9 School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh, EH9 3FJ, UK. [email protected]
  • 10 Department of Analytical Chemistry, Faculty of Pharmacy, Izmir Katip Celebi University, Izmir, Turkey. [email protected] , (Turkey)
  • 11 Centre for Photonics and Photonic Materials, Department of Physics, University of Bath, Bath, BA2 7AY, UK. [email protected]
Type
Published Article
Journal
European Journal of Nuclear Medicine
Publisher
Springer-Verlag
Publication Date
Mar 01, 2021
Volume
48
Issue
3
Pages
800–807
Identifiers
DOI: 10.1007/s00259-020-05021-4
PMID: 32915268
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The relentless rise in antimicrobial resistance is a major societal challenge and requires, as part of its solution, a better understanding of bacterial colonization and infection. To facilitate this, we developed a highly efficient no-wash red optical molecular imaging agent that enables the rapid, selective, and specific visualization of Gram-positive bacteria through a bespoke optical fiber-based delivery/imaging endoscopic device. We rationally designed a no-wash, red, Gram-positive-specific molecular imaging agent (Merocy-Van) based on vancomycin and an environmental merocyanine dye. We demonstrated the specificity and utility of the imaging agent in escalating in vitro and ex vivo whole human lung models (n = 3), utilizing a bespoke fiber-based delivery and imaging device, coupled to a wide-field, two-color endomicroscopy system. The imaging agent (Merocy-Van) was specific to Gram-positive bacteria and enabled no-wash imaging of S. aureus within the alveolar space of whole ex vivo human lungs within 60 s of delivery into the field-of-view, using the novel imaging/delivery endomicroscopy device. This platform enables the rapid and specific detection of Gram-positive bacteria in the human lung.

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