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Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar

Authors
  • Yang, Zhiyuan1, 2
  • Murata, Shiro1
  • Fujisawa, Sotaro1
  • Takehara, Masaki1
  • Katakura, Ken1
  • Hmoon, Myint Myint3
  • Win, Shwe Yee3
  • Bawm, Saw3
  • Konnai, Satoru1
  • Ohashi, Kazuhiko1
  • 1 Hokkaido University, Sapporo, Japan , Sapporo (Japan)
  • 2 Beijing Academy of Agriculture and Forestry Sciences, Beijing, China , Beijing (China)
  • 3 University of Veterinary Science, Yezin, Nay Pyi Taw, Myanmar , Nay Pyi Taw (Myanmar (Burma))
Type
Published Article
Journal
BMC Veterinary Research
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Nov 23, 2020
Volume
16
Issue
1
Identifiers
DOI: 10.1186/s12917-020-02666-z
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundAvian infectious laryngotracheitis (ILT) is a highly contagious viral disease that causes severe economic losses to the poultry industry worldwide. In Southeast Asian countries, including Myanmar, poultry farming is a major industry. Although it is known that infectious respiratory pathogens, including infectious laryngotracheitis virus (ILTV), are a major threat to poultry farms, there are no data currently available on the epidemiology of ILTV in Myanmar. Therefore, in this study, we conducted a molecular detection of ILTV in 20 poultry farms in Myanmar.ResultsOf the 57 tested oropharyngeal swabs, 10 were positive for ILTV by polymerase chain reaction of a 647 bp region of the thymidine kinase (TK) gene, giving a prevalence of ILTV of 17.5% (10/57). Further sequencing analysis of infected cell protein 4 (ICP4) gene and glycoprotein B, G, and J (gB, gG, and gJ) genes indicated that these isolates were field strains. Phylogenetic analysis revealed that the Myanmar strains clustered together in a single branch and were closely related to other reference strains isolated from Asian countries.ConclusionsThis study demonstrated the presence of ILTV in poultry farms in Myanmar. The genetic characterization analysis performed provides the fundamental data for epidemiological studies that monitor circulating strains of ILTV in Myanmar.

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