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Molecular cloning and mRNA expression pattern of Sox\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\varvec{Sox}$$\end{document}4 in Misgurnus anguillicaudatus

Authors
  • Xia, Xiaohua1
  • Wan, Ruyan1
  • Huo, Weiran1
  • Zhang, Linxia1
  • Xia, Xiaopei1
  • Chang, Zhongjie1
  • 1 Henan Normal University, College of Life Science, Xinxiang, Henan, 453007, People’s Republic of China , Xinxiang (China)
Type
Published Article
Journal
Journal of Genetics
Publisher
Springer-Verlag
Publication Date
Aug 14, 2018
Volume
97
Issue
4
Pages
869–877
Identifiers
DOI: 10.1007/s12041-018-0972-2
Source
Springer Nature
Keywords
License
Yellow

Abstract

Sox4, a member of the SoxC subfamily which of the Sox family, plays important roles in the development of the vertebrate gonad and nervous system. We have cloned a Sox4 homologue from the brain of Misgurnus anguillicaudatus using homologous cloning and rapid amplification of cDNA ends. We named the cloned gene as MaSox4. The full-length cDNA was 2122 bp, containing a 718 bp 5′\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$5^{\prime }$$\end{document}-untranslated region and a 267 bp 3′\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$3^{\prime }$$\end{document}-untranslated region. The open-reading frame of the cloned gene encoded 378 amino acids and contained a characteristic HMG-box DNA-binding domain with the specific motif (RPMNAFMVW). Phylogenetic analysis indicated that MaSox4 is highly homologous to Sox4 in different species. Protein sequence analysis showed that MaSox4 is a nonsecretory hydrophilic protein. Quantitative real-time reverse transcription polymerase chain reaction and in situ hybridization assay revealed that MaSox4 was ubiquitously expressed during embryogenesis and is present in various adult tissues, especially in the central nervous system. Our study suggests that MaSox4 is highly conserved among vertebrates’ evolution and might be involved in developmental processes such as embryogenesis, neurogenesis and gonad development.

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