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Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.

Authors
  • S Cotecchia
  • D A Schwinn
  • R R Randall
  • R J Lefkowitz
  • M G Caron
  • B K Kobilka
Publication Date
Oct 01, 1988
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.

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