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Molecular characterization of an inhibitor of apoptosis protein (IAPs) in freshwater pearl mussel, Hyriopsis schlegelii.

Authors
  • Wu, Di1
  • Wang, Chengyuan1, 2
  • Zhang, Wanchang1
  • Peng, Kou1
  • Sheng, Junqing1
  • Wang, Junhua1
  • Jain, Archana3
  • Hong, Yijiang1, 4
  • 1 School of Life Sciences, Nanchang University , Nanchang , China. , (China)
  • 2 Jiangzhong dietary therapy technology Co. LTD , Jiangxi , China. , (China)
  • 3 Key Laboratory of Basic Pharmacology and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University , Guizhou , China. , (China)
  • 4 Key Lab of Aquatic Resources and Utilization of Jiangxi , Nanchang , China. , (China)
Type
Published Article
Journal
Bioengineered
Publisher
Landes Bioscience
Publication Date
Dec 01, 2019
Volume
10
Issue
1
Pages
365–373
Identifiers
DOI: 10.1080/21655979.2019.1653738
PMID: 31446833
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The inhibitor of apoptosis proteins (IAPs) played important roles in inhibiting the apoptosis of tumor cells by regulating caspase activity in mammals. In this study, we first cloned the full-length cDNA sequence of IAPs gene (designated as Hs-IAPs) in Hyriopsis schlegelii. The Hs-IAPs gene contained an open reading frame of 1719 nucleotides, encoding a predicted protein of 572 amino acids. qRT-PCR assay indicated that the Hs-IAPs gene was ubiquitously expressed in different tissues, and the highest expression level was in gills. Furthermore, we purified and obtained the recombinant protein of Hs-IAPs which showed a molecular weight of 82.5 kDa. We used H2O2 stimulation experiment to explore the possible function of Hs-IAPs. The results showed that the percentage of viable cells significantly increased following the Hs-IAPs concentration. These indicated that the Hs-IAPs may play a role in anti-oxidation causing by H2O2, and its anti-oxidative may be crucial in the process of apoptosis.

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