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Molecular characterization of Arabidopsis PHO80-like proteins, a novel class of CDKA;1-interacting cyclins

Authors
  • Torres Acosta, J. A.1
  • de Almeida Engler, J.2
  • Raes, J.3
  • Magyar, Z.4
  • De Groodt, R.3
  • Inzé, D.3
  • De Veylder, L.3
  • 1 University of Agricultural Sciences, Center of Applied Genetics, Muthgasse 18, Vienna, 1190, Austria , Vienna
  • 2 Unité Interactions Plantes-Microorganismes et Santé Végétale, Institut National de la Recherche Agronomique, Antibes Cedex, 06606, France , Antibes Cedex
  • 3 Ghent University, Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Technologiepark 927, Gent, 9052, Belgium , Gent
  • 4 University of London, School of Biological Sciences, Royal Holloway, Eghem, TW20 QEX, United Kingdom , Eghem
Type
Published Article
Journal
Cellular and Molecular Life Sciences
Publisher
Springer-Verlag
Publication Date
Jun 01, 2004
Volume
61
Issue
12
Pages
1485–1497
Identifiers
DOI: 10.1007/s00018-004-4057-4
Source
Springer Nature
Keywords
License
Yellow

Abstract

Cyclins are regulatory proteins that interact with cyclin-dependent kinases (CDKs) to control progression through the cell cycle. In Arabidopsis thaliana, 34 cyclin genes have been described, grouped into five different types (A, B, D, H, and T). A novel class of seven cyclins was isolated and characterized in Arabidopsis, designated P-type cyclins (CYCPs). They all share a conserved central region of 100 amino acids (“cyclin box”) displaying homology to the corresponding region of the PHO80 cyclin from Saccharomyces cerevisiae and the related G1 cyclins from Trypanosoma cruzi and T. brucei. The CYCP4;2 gene was able to partially re-establish the phosphate-dependent expression of the PHO5 gene in a pho80 mutant strain of yeast. The CYCPs interact preferentially with CDKA;1 in vivo and in vitro as shown by yeast two-hybrid analysis and co-immunoprecipitation experiments. P-type cyclins were mostly expressed in proliferating cells, albeit also in differentiating and mature tissues. The possible role of CYCPs in linking cell division, cell differentiation, and the nutritional status of the cell is discussed.

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