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Molecular and Biochemical Characterization of a Cytokinin Oxidase from Maize1

Authors
  • Kristin D. Bilyeu
  • Jean L. Cole
  • James G. Laskey
  • Wayne R. Riekhof
  • Thomas J. Esparza
  • Michelle D. Kramer
  • Roy O. Morris
Publisher
American Society of Plant Physiologists
Publication Date
Jan 01, 2001
Source
PMC
Keywords
Disciplines
  • Biology
  • Chemistry
License
Unknown

Abstract

Molecular and Biochemical Characterization of a Cytokinin Oxidase from Maize by Kristin D. Bilyeu, Jean L. Cole, James G. Laskey, Wayne R. Riekhof, Thomas J. Esparza, Michelle D. Kramer, and Roy O. Morris Plantphysiol Volume 125(1):378-386 January 1, 2001 ©2001 by American Society of Plant Biologists Genomic organization of the maize and Arabidopsis cytokinin oxidase genes. Bilyeu K et al. Plantphysiol 2001;125:378-386 ©2001 by American Society of Plant Biologists Expression of recombinant maize cytokinin oxidase. Bilyeu K et al. Plantphysiol 2001;125:378-386 ©2001 by American Society of Plant Biologists Inhibition of cytokinin oxidase activity by CPPU. No CPPU (●), 1.5 μm CPPU (▴), and 3 μM CPPU (▪). Bilyeu K et al. Plantphysiol 2001;125:378-386 ©2001 by American Society of Plant Biologists Identification of flavin cofactor. Bilyeu K et al. Plantphysiol 2001;125:378-386 ©2001 by American Society of Plant Biologists Distribution of glycosylated cytokinin oxidase in maize. Bilyeu K et al. Plantphysiol 2001;125:378-386 ©2001 by American Society of Plant Biologists Genomic organization of the maize and Arabidopsis cytokinin oxidase genes. Restriction map and gene structure of maizeckx1 and gene structures of seven Arabidopsis cytokinin oxidase homologs. Exons (numbered rectangles) and introns (solid and dashed lines, not to scale) are indicated. Restriction enzymes: N,NotI; H, HindIII; and B, BamHI. Amino acid identity is for all exons of each gene compared with maizeckx1 exons. Oxidase activity is that exhibited by P. pastoris supernatants expressing the appropriate cDNA. ND, Gene activity not determined. *, All introns were predicted; **, first intron was predicted. Expression of recombinant maize cytokinin oxidase. A, Time course of expression of maize ckx1 in P. pastoris. Black bars, Cytokinin oxidase activity in P. pastoris supernatants expressing maize ckx1 (introns removed) with its native signal peptide (MAVVYYLLLA GLIACSHA-LAA); cross-hatched bars, cytokinin oxidase activi

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