Molecular analysis of two local falciparum malaria outbreaks on the French Guiana coast confirms the msp1 B-K1/varD genotype association with severe malaria

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Molecular analysis of two local falciparum malaria outbreaks on the French Guiana coast confirms the msp1 B-K1/varD genotype association with severe malaria

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BioMed Central
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PMC
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  • Biology
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Abstract

1475-2875-4-26.fm ral ss BioMed CentMalaria Journal Open AcceResearch Molecular analysis of two local falciparum malaria outbreaks on the French Guiana coast confirms the msp1 B-K1/varD genotype association with severe malaria Eric Legrand*1, Beatrice Volney1, Anne Lavergne1, Caroline Tournegros1, Loïc Florent1, Doris Accrombessi1, Micheline Guillotte2, Odile Mercereau- Puijalon2 and Philippe Esterre1 Address: 1Centre National de Référence sur la Chimiorésistance du Paludisme dans la région Antilles – Guyane, Institut Pasteur de la Guyane, BP 6010, F-97306 Cayenne-Cedex, France and 2Unité d'Immunologie Moléculaire des Parasites, Unité de Recherche Associée 2581 du Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France Email: Eric Legrand* - [email protected]; Beatrice Volney - [email protected]; Anne Lavergne - [email protected] cayenne.fr; Caroline Tournegros - [email protected]; Loïc Florent - [email protected]; Doris Accrombessi - [email protected] cayenne.fr; Micheline Guillotte - [email protected]; Odile Mercereau-Puijalon - [email protected]; Philippe Esterre - [email protected] cayenne.fr * Corresponding author Abstract Background: Plasmodium falciparum outbreaks can occur in the coastal area of French Guiana, where the population is essentially non-immune. Two sporadic outbreaks were observed, including one with severe malaria cases. To characterize these outbreaks and verify previous observations of specific genotype characteristics in severe malaria in this area, all cases from each outbreak were studied. Methods: P. falciparum genotypes for six genetic loci were determined by PCR amplification from peripheral blood parasites. The msp1/block2 and msp2 genotypes were determined by DNA sequencing. Microsatellite and varD genotyping was based on size polymorphism and locus-specific amplification. Results: The outbreak including severe malaria cases was associated with a single genotype. The other mild mala

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