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Molecular analysis of the TyrR protein-mediated activation of mtr gene expression in Escherichia coli K-12.

Authors
  • J P Sarsero
  • A J Pittard
Publication Date
Dec 01, 1991
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

Expression of the mtr gene, which encodes a tryptophan-specific transport system in Escherichia coli K-12, is activated by the TyrR protein. Two TyrR protein binding sites (TYR R boxes) are positioned upstream of the -35 promoter region. Mutational and DNase protection studies indicate that TyrR protein binds preferentially to the TYR R box closest to the promoter, and this is essential for activation of gene expression. In the presence of tyrosine and ATP, a second TyrR molecule is able to cooperatively bind to the second box and cause a further increase in the level of activation.

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