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Molecular analysis of mammalian timeless.

Authors
  • Zylka, M J
  • Shearman, L P
  • Levine, J D
  • Jin, X
  • Weaver, D R
  • Reppert, S M
Type
Published Article
Journal
Neuron
Publisher
Elsevier
Publication Date
Nov 01, 1998
Volume
21
Issue
5
Pages
1115–1122
Identifiers
PMID: 9856466
Source
Medline
License
Unknown

Abstract

We cloned the mouse cDNA of a mammalian homolog of the Drosophila timeless (tim) gene and designated it mTim. The mTim protein shows five homologous regions with Drosophila TIM. mTim is weakly expressed in the suprachiasmatic nuclei (SCN) but exhibits robust expression in the hypophyseal pars tuberalis (PT). mTim RNA levels do not oscillate in the SCN nor are they acutely altered by light exposure during subjective night. mTim RNA is expressed at low levels in several peripheral tissues, including eyes, and is heavily expressed in spleen and testis. Yeast two-hybrid assays revealed an array of interactions between the various mPER proteins but no mPER-mTIM interactions. The data suggest that PER-PER interactions have replaced the function of PER-TIM dimers in the molecular workings of the mammalian circadian clock.

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