Antibodies against calcium-binding protein S100 (AB-S100) 1.5-fold increased, while quinine 2-fold decreased the frequency of action potential generation in B4 and B6 neurons. Application of quinine against the background of AB-S100 treatment returned this parameter to the initial level. Pretreatment with AB-S100 in low doses prevents changes the frequency of action potential generation induced by application of AB-S100 in the initial dilution. The duration of action potential increased by 1.6 times after application of AB-S100 and quinine, while after application of quinine increased this parameter by more than 6 times. AB-S100 decreased maximum inward current by 20%. Our experiments demonstrated a modulating effect of combined administration of AB-S100 and its low doses on the membrane effects of quinine.