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Modulation of the gene expression in the protective effects of electroacupuncture against cerebral ischemia: a cDNA microarray study.

Authors
  • Guo, Jing-Chun
  • Gao, Huan-min
  • Chen, Jian
  • Zhao, Peng
  • Cao, Xiao-ding
  • Li, Yao
  • Cheng, Jie-shi
Type
Published Article
Journal
Acupuncture & Electro-Therapeutics Research
Publisher
"Cognizant, LLC"
Publication Date
Jan 01, 2004
Volume
29
Issue
3-4
Pages
173–186
Identifiers
PMID: 15807099
Source
Medline
License
Unknown

Abstract

In the present study, a cDNA microarray technology was used to analyze the gene expression profile in ischemia and in electroacupuncture (EA)-treated ischemia. A focal cerebral ischemia/reperfusion model on Macaca mulatta was performed with a modified middle cerebral artery occlusion method. Among the nearly 8000 genes, approximately 8% of the total number of genes examined were affected after ischemia/reperfusion injury. Major altered genes were downregulated. In EA-treated monkeys, approximately 10% of the total number of genes examined were affected. Major altered genes were upregulated, including signal transduction-, cell-cycle-, metabolism-, stress response-, DNA repair-related genes. One of the representative upregulated genes encodes insulin-like growth factor-1 (IGF-1) was confirmed using in situ hybridization. Results showed that after ischemia/reperfusion injury, IGF-1 mRNA expression decreased in ipsilateral striatum, whereas increased in ipsilateral hippocampus. No expression changes were observed in cortex. EA treatment could obviously upregulate the IGF-1 mRNA expression in striatum, and further enhance its expression in hippocampus. Therefore, the data presented suggest a possible mediator underlying the mechanisms of anti-ischemic effect of acupuncture. In conclusion, the protective mechanisms of EA against stroke include several related pathways and gene expressions. Microarray analysis may provide a framework for understanding these complicated mechanisms and yield valuable, clinically relevant insights and potentially therapeutic targets of stroke.

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