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Modular assembly of protein building blocks to create precisely defined megamolecules.

Authors
  • Modica, Justin A
  • Skarpathiotis, Stratos
  • Mrksich, Milan
Type
Published Article
Journal
ChemBioChem
Publisher
Wiley (John Wiley & Sons)
Publication Date
Nov 05, 2012
Volume
13
Issue
16
Pages
2331–2334
Identifiers
DOI: 10.1002/cbic.201200501
PMID: 23070998
Source
Medline
License
Unknown

Abstract

Enzyme-promoted assembly: The construction of a hetero-bifunctional protein building block, HaloTag-cutinase, that reacts rapidly and selectively with a small-molecule linker is described. The step-wise combination of these building blocks generates a 300 kDa "megamolecule" with precisely defined domain orientation, connectivity, and composition.

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