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Modified methodology to improve flow cytometric DNA histograms from paraffin-embedded material.

Authors
  • McLemore, D D
  • el Naggar, A
  • Stephens, L C
  • Jardine, J H
Type
Published Article
Journal
Stain technology
Publication Date
Jan 01, 1990
Volume
65
Issue
6
Pages
279–291
Identifiers
PMID: 2080523
Source
Medline
License
Unknown

Abstract

Flow Cytometric (FCM) DNA content analysis of paraffin-embedded tissues has become a widely accepted procedure in assessing the biologic course in some tumors from archival material. Difficulty in interpreting histograms is frequently due to high levels of debris, and wide coefficients of variation (CV). These may lead to underestimating near-diploid abnormality. Although the clinical significance of low-degree aneuploidy has yet to be established, the procedure reported here improved our endeavor to detect DNA Indices (DI) of at least 1.1%. Experience has shown that careful technique can result in overall improvement of DNA histograms by lowering levels of debris and % CV, dramatically so in some cases. Archival histograms can be generated that rival in quality fresh tissue results. Archival material is currently employed for diagnostic and research purposes.

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