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A modified method for obtaining large amounts of high titer polyclonal ascites fluid.

Authors
  • Ou, S K
  • Hwang, J M
  • Patterson, P H
Type
Published Article
Journal
Journal of Immunological Methods
Publisher
Elsevier
Publication Date
Sep 27, 1993
Volume
165
Issue
1
Pages
75–80
Identifiers
PMID: 8409470
Source
Medline
License
Unknown

Abstract

When limited amounts of antigens are available, it is often difficult to obtain large quantities of polyclonal antisera. Although antisera can be raised in small rodents, yields are usually small. To circumvent these problems, we have designed a modified method for generating polyclonal ascites fluid (AF). Using the appropriate strain of mice and adjuvant, we generated high serum titers by injection of 1-100 micrograms of protein. Following i.p. injection of compatible sarcoma cells, 13-19 ml of high titer (1:1000-1:20,000), polyclonal ascites fluid were obtained from each mouse. Similar results were obtained using nine different antigens.

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