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A modified high-performance thin-layer plate for the separation of purines and pyrimidines.

Authors
  • Jost, W
  • Hauck, H E
Type
Published Article
Journal
Analytical biochemistry
Publication Date
Nov 01, 1983
Volume
135
Issue
1
Pages
120–127
Identifiers
PMID: 6670735
Source
Medline
License
Unknown

Abstract

Several ways of using the recently developed high-performance thin-layer chromatography (HPTLC) precoated plate NH2 F 254s to separate purines and pyrimidines are described. This precoated plate is coated with silica gel 60 which has been chemically modified with alkylamino groups. In view of the chemical properties of the functional groups bonded to the silica gel matrix, the HPTLC precoated plate NH2 F 254s can be considered to be a weak basic ion-exchange plate. In aqueous eluants the substances are separated principally according to charge differences. The HPTLC precoated plate NH2 F 254s can, however, also be used to separate uncharged, polar compounds with organic solvents. Examples of separations and chromatograms for its use in both aqueous and organic eluants are given.

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