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Modified bacterial artificial chromosomes for zebrafish transgenesis.

Authors
Type
Published Article
Journal
Methods (San Diego, Calif.)
Publication Date
Volume
39
Issue
3
Pages
183–188
Identifiers
PMID: 16828309
Source
Medline
License
Unknown

Abstract

Transgenesis using bacterial artificial chromosomes (BAC) offers greater fidelity in directing desirable expression of foreign genes. Application of this technology in the optically transparent zebrafish with fluorescent protein reporters enables unparalleled visual analysis of regulation of gene expression in a living organism. Here we describe a streamlined procedure of direct selecting multiple BAC clones based on public sequence databases followed by rapid modification with GFP or RFP for transgenic analysis in zebrafish. Experimental procedures for BAC DNA preparation, microinjection of zebrafish embryos and screening of transgenic zebrafish carrying GFP/RFP modified BAC clones are detailed.

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