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A modification of the rapid Golgi method.

Authors
  • Kemali, M
Type
Published Article
Journal
Stain technology
Publication Date
May 01, 1976
Volume
51
Issue
3
Pages
169–172
Identifiers
PMID: 779149
Source
Medline
License
Unknown

Abstract

An extremely rapid Golgi procedure for immersion impregnation of the whole brain of the frog Rana esculenta is described. It uses an isotonic 2% osmium tetroxide solution buffered with sodium barbital at pH 7.2 that has previously been used for electron microscopy. To 15 ml of this solution 85 ml 3% potassium dichromate is added just before use. Twelve-hour treatments (6 hr in the chrome-osmium solution plus 6 hr in 0.75% silver nitrate) are sufficient to obtain consistently excellent impregnations of external brain formations. Twenty-four-hour treatments (12 hr in the chrome-osmium solution and 12 hr in the silver solution) impregnate also the deeper regions of the brain. The results show neuronal details on a clear background.

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