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Modeling human hepatic steatosis in pluripotent stem cell-derived hepatocytes.

Authors
  • Sinton, Matthew C1
  • Meseguer-Ripolles, Jose2
  • Lucendo-Villarin, Baltasar2
  • Drake, Amanda J1
  • Hay, David C2
  • 1 University/BHF Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute, Edinburgh BioQuarter, 47 Little France Crescent, Edinburgh EH16 4TJ, UK. , (France)
  • 2 Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, Edinburgh BioQuarter, 5 Little France Crescent, Edinburgh EH16 4UU, UK. , (France)
Type
Published Article
Journal
STAR protocols
Publication Date
Jun 18, 2021
Volume
2
Issue
2
Pages
100493–100493
Identifiers
DOI: 10.1016/j.xpro.2021.100493
PMID: 33997813
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This protocol describes the production of hepatocyte-like cells (HLCs) from human pluripotent stem cells and how to induce hepatic steatosis, a condition characterized by intracellular lipid accumulation. Following differentiation to an HLC phenotype, intracellular lipid accumulation is induced with a steatosis induction cocktail, allowing the user to examine the cellular processes that underpin hepatic steatosis. Furthermore, the renewable nature of our system, on a defined genetic background, permits in-depth mechanistic analysis, which may facilitate therapeutic target identification in the future. For complete details on the use and execution of this protocol, please refer to Sinton et al. (2021). © 2021 The Author(s).

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