Affordable Access

deepdyve-link
Publisher Website

Model Compounds Study for the Mechanism of Horseradish Peroxidase-Catalyzed Lignin Modification.

Authors
  • Yang, Dongjie1, 2
  • Wang, Yalin1, 2
  • Huang, Wenjing1, 2
  • Li, Zhixian3, 4
  • Qiu, Xueqing5, 6
  • 1 School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China. , (China)
  • 2 State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China. , (China)
  • 3 School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China. [email protected] , (China)
  • 4 State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China. [email protected] , (China)
  • 5 School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China. [email protected] , (China)
  • 6 State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China. [email protected] , (China)
Type
Published Article
Journal
Applied Biochemistry and Biotechnology
Publisher
Springer-Verlag
Publication Date
Jul 01, 2020
Volume
191
Issue
3
Pages
981–995
Identifiers
DOI: 10.1007/s12010-020-03248-3
PMID: 31950443
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Horseradish peroxidase (HRP) has demonstrated high activity for the modification of lignin. In this paper, several lignin model compounds with different functional groups and linkages are selected to investigate the reactivity of HRP-catalyzed lignin modification. The phenolic groups of lignin model compounds are indispensable for the HRP-catalyzed modification process. The introduction of the sulfomethylated methyl group or methoxyl group could facilitate or inhibit the modification, respectively. The oxidative coupling activity of α-O-4 lignin model compounds is higher than that of β-O-4 compounds. Meanwhile, the free energy obtained by density functional theory (DFT) is used to verify the results of the experimental study, and the order of preference for linkages is β-5 > β-β > β-O-4 in most cases. In addition, electron cloud density and steric hindrance of lignin model compounds have crucial effects on the oxidation and modification processes. Finally, the mechanism of HRP-catalyzed lignin modification is proposed.

Report this publication

Statistics

Seen <100 times