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Mitochondrial GFA2 Is Required for Synergid Cell Death in Arabidopsis

Authors
  • Cory A. Christensen
  • Steven W. Gorsich
  • Ryan H. Brown
  • Linda G. Jones
  • Jessica Brown
  • Janet M. Shaw
  • Gary N. Drews
Publisher
American Society of Plant Biologists
Publication Date
Sep 01, 2002
Source
PMC
Keywords
Disciplines
  • Chemistry
License
Unknown

Abstract

Mitochondrial GFA2 Is Required for Synergid Cell Death in Arabidopsis by Cory A. Christensen, Steven W. Gorsich, Ryan H. Brown, Linda G. Jones, Jessica Brown, Janet M. Shaw, and Gary N. Drews Plantcell Volume 14(9):2215-2232 September 1, 2002 ©2002 by American Society of Plant Biologists Developmental Stages Affected in the FG Mutants.Megagametogenesis has been described previously and divided into seven stages (Christensen et al., 1997). Christensen C et al. Plantcell 2002;14:2215-2232 ©2002 by American Society of Plant Biologists Phenotypic Analysis of the FG Mutants.(A) and (B) Wild-type FG at the terminal developmental stage (stage FG7). Christensen C et al. Plantcell 2002;14:2215-2232 ©2002 by American Society of Plant Biologists GFA2 Gene Sequence.Exons are indicated with uppercase letters. Christensen C et al. Plantcell 2002;14:2215-2232 ©2002 by American Society of Plant Biologists Alignment of the Predicted Protein Sequences for Arabidopsis GFA2, Yeast Mdj1p, and E. coli DnaJ.The J domain (residues 90 to 156) and the zinc-finger domain (residues 238 to 294) are double underlined. Christensen C et al. Plantcell 2002;14:2215-2232 ©2002 by American Society of Plant Biologists Reverse Transcriptase –PCR Analysis of GFA2 Expression. Christensen C et al. Plantcell 2002;14:2215-2232 ©2002 by American Society of Plant Biologists GFA2::GUS Expression in the Wild Type, GFA2-GFP Protein Localization in the Wild Type, and FIE::FIE-sGFP Expression in the gfa2 Mutant.(A) and (B) Histochemical assay for the expression of the GFA2::GUS transgene in the whole plant (A) and the flower (B). Christensen C et al. Plantcell 2002;14:2215-2232 ©2002 by American Society of Plant Biologists GFA2 Complementation of the Yeast mdj1 Mutant Growth Defect.Cultures of the four possible combinations of the MDJ1 or mdj1::HIS3 allele and the expression vectors pRS416-MET25-GFA2 or pRS416-MET25 were diluted serially (1:10) and grown at 25, 30, and 37°C o... Christensen C et al. Plantcell 2002;14:2215-2

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