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MISpheroID : a knowledgebase and transparency tool for minimum information in spheroid identity

Authors
  • Peirsman, Arne
  • Blondeel, Eva
  • Ahmed, Tasdiq
  • Anckaert, Jasper
  • Audenaert, Dominique
  • Boterberg, Tom
  • Buzas, Krisztina
  • Carragher, Neil
  • Castellani, Gastone
  • Castro, Flávia
  • Dangles-Marie, Virginie
  • Dawson, John
  • De Tullio, Pascal
  • De Vlieghere, Elly
  • Dedeyne, Sandor
  • Depypere, Herman
  • Diosdi, Akos
  • Dmitriev, Ruslan
  • Dolznig, Helmut
  • Fischer, Suzanne
  • And 31 more
Publication Date
Jan 01, 2021
Identifiers
DOI: 10.1038/s41592-021-01291-4
OAI: oai:archive.ugent.be:8725528
Source
Ghent University Institutional Archive
Keywords
Language
English
License
Green
External links

Abstract

Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empirical evaluation and interlaboratory validation of selected variations in spheroid methodology revealed diverse impacts on spheroid metrics. To facilitate interpretation, stimulate transparency and increase awareness, the Consortium defines the MISpheroID string, a minimum set of experimental parameters required to report spheroid research. Thus, MISpheroID combines a valuable resource and a tool for three-dimensional cellular models to mine experimental parameters and to improve reproducibility.

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