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MiR-608 Exerts Anti-inflammatory Effects by Targeting ELANE in Monocytes.

Authors
  • Gu, Wei1
  • Wen, Dalin2, 3
  • Lu, Hongxiang3
  • Zhang, Anqiang3
  • Wang, Haiyan3
  • Du, Juan3
  • Zeng, Ling3
  • Jiang, Jianxin4
  • 1 School of Medicine, Changing University, Shapingba, Chongqing, 400045, China. [email protected] , (China)
  • 2 Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, School of Medicine, Chongqing University, Shapingba, Chongqing, 400045, China. , (China)
  • 3 State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University, Daping, Chongqing, 400042, China. , (China)
  • 4 State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University, Daping, Chongqing, 400042, China. [email protected] , (China)
Type
Published Article
Journal
Journal of Clinical Immunology
Publisher
Springer-Verlag
Publication Date
Nov 20, 2019
Identifiers
DOI: 10.1007/s10875-019-00702-8
PMID: 31749032
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

miR-608 has been indicated to play an important role in the pathogenesis of various inflammation-related diseases, including sepsis and several types of cancers. However, there is little information about the underlying mechanism, especially in inflammatory cells. In this study, an hsa-miR-608-inhibition cell model was constructed in U937 cells using a lentivirus, and gene expression profiles were determined by a cDNA microarray. Altogether, 682 genes showed a difference greater than 1.2-fold, including 184 genes downregulated and 498 genes upregulated. Among these genes, one potential miR-608-target gene, ELANE, was further investigated. A positive relationship between the expression of miR-608 and that of ELANE was found both in vivo and in vitro. In addition, decreased expression of miR-608 resulted in overexpression of ELANE at both the mRNA and protein levels. Cotransfection of HEK293T cells with a miR-608 mimic inhibited reporter activity, and mutation of the miRNA seed sequences abolished the repression of reporter activity. These results suggest that miR-608 is an important posttranscriptional regulator of ELANE expression in human monocytes and may play an important role in the process of inflammation. miR-608 and neutrophil elastase may be novel targets for the diagnosis or treatment of sepsis.

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