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MiR-499 inhibited hypoxia/reoxygenation induced cardiomyocytes injury by targeting SOX6

Authors
  • Shi, Yujie1
  • Han, Yunfeng2
  • Niu, Lili2
  • Li, Junxia2
  • Chen, Yundai1
  • 1 Chinese PLA General Hospital, Department of Cardiology, No. 28 Fuxing Road, Beijing, 100853, China , Beijing (China)
  • 2 PLA Army General Hospital, Cardiovascular Disease Institute, Beijing, China , Beijing (China)
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
May 10, 2019
Volume
41
Issue
6-7
Pages
837–847
Identifiers
DOI: 10.1007/s10529-019-02685-3
Source
Springer Nature
Keywords
License
Green

Abstract

ObjectiveMiR-499 has been reported to be expressed only in cardiomyocytes, and its expression would increase after acute myocardial infarction (AMI). miR-499 plays a role in the process of cardiomyocytes injury induced by hypoxia/reoxygenation (H/R), however, it still remains unclear.ResultsHypoxia inhibited miR-499-5p expression and H/R induced apoptosis. SOX6 was a target gene of miR-499-5p, and high expression of miR-499-5p inhibited the expression of SOX6. MiR-499-5p reduced H9c2 cells injury by inhibiting the expression of SOX6, overexpression of which could reverse the effect of miR-499-5p on H9c2 cells. MiR-499-5p inhibited the levels of LDH and MDA, while overexpression of miR-499-5p inhibited H/R-induced cell apoptosis. MiR-499-5p could up-regulate the level of Bcl-2 and down-regulate the expression levels of Bax and caspase-3. However, SOX6 partially reversed these effects of miR-499-5p.ConclusionWe proved that miR-499-5p inhibited H/R-induced cardiomyocytes injury by targeting SOX6. Our results suggested that miR-499-5p/SOX6 pathway may present a potential therapeutic target for the treatment of AMI.

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