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miR-497-5p induces apoptosis in K562 cells by downregulating ROCK1

Authors
  • Chen, Nafei1
  • Meng, Zhen2
  • Song, Jiaojie1
  • Kong, Lingfang1
  • Zhang, Yehua1
  • Guo, Suli1
  • Zhang, Xiaokun1
  • Lu, Xin1
  • Jiang, Licai1
  • Chen, Ran1
  • Jiao, Zongjiu1
  • Zhao, Liyun1
  • 1 Department of Hematology, Xingtai People’s Hospital, Xingtai 054000, Hebei Province, China
  • 2 Department of Hematology, Hudson International Peace Hospital, Heng Shui City People’s Hospital, Hengshui 053000, Hebei Province, China
Type
Published Article
Journal
American journal of translational research
Publisher
Madison, WI : e-Century Pub. Corp.
Publication Date
Aug 15, 2021
Volume
13
Issue
8
Pages
9278–9284
Identifiers
PMID: 34540044
PMCID: PMC8430193
Source
PubMed Central
Keywords
Disciplines
  • Original Article
License
Unknown

Abstract

Objective: To validate the role of miR-497-5p in apoptosis in K562 cells by targeting Rho-associated kinase isoform 1 (ROCK1). Methods: From January, 2017 to February, 2019, 57 patients with chronic myeloid leukemia (CML) treated in our hospital were included in patient group, and 50 healthy individuals were recruited as control group. miR-497-5p level in peripheral blood was quantitated using qRT-PCR. After transfecting with miR-497-5p overexpression vector and ROCK1 inhibitor, K562 cells were monitored in terms of proliferation (CCK8 assay), migration and invasion (Transwell), and apoptosis (flow cytometry). Binding loci between miR-497-5p and ROCK1 were predicted, and the targeting relationship was confirmed (dual-luciferase reporter (DLR) assay). Results: miR-497-5p was poorly expressed in CML ( P < 0.05). Forced overexpression of miR-497-5p or inhibition of ROCK1 suppressed malignant processes (proliferation, proliferation, migration and invasion) in K562 cells and induced apoptosis ( P < 0.05). DLR assay revealed a decreased luciferase activity after miR-497-5p binding to ROCK1 ( P < 0.05). Conclusion: miR-497-5p induces apoptosis in K562 cells by downregulating ROCK1.

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