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miR-219a-1 inhibits colon cancer cells proliferation and invasion by targeting MEMO1

  • Xu, Keqing1
  • Shi, Jie
  • Mo, Dongping2
  • Yang, Yanhua1
  • Fu, Qiang
  • Luo, Ying3
  • 1 Department of Comprehensive Medical Laboratory, Changzhou No. 7 People’s Hospital, P. R. China
  • 2 Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, P. R. China
  • 3 Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai
Published Article
Cancer Biology & Therapy
Landes Bioscience
Publication Date
Nov 20, 2020
DOI: 10.1080/15384047.2020.1843897
PMID: 33218285
PMCID: PMC7722797
PubMed Central


Colon cancer is the third most common cancer worldwide. Many miRNAs have been reported to be involved in colon cancer progression. However, there are only a few studies on the role of miR-219a-1 in colon cancer, and the molecular mechanisms involved remain unclear. The aim of this study was to investigate the miR-219a-1 level in patients with colon cancer and to explore both the effects and regulatory mechanisms of miR-219a-1 in the malignancy of colon cancer cells. Real-time PCR and western blot analysis were used to analyze the expression levels of miR-219a-1 and mediator of ErbB2-driven cell motility 1. Cell Counting Kit-8, transwell and wound-healing assays were performed to investigate the malignant ability of colon cancer cells. A luciferase assay was performed to explore whether miR-219a-1 could directly bind to 3ʹ-UTR region of MEMO1 . miR-219a-1 was found to be downregulated in colon cancer cell lines and in patients with colon cancer. Additionally, miR-219a-1 could inhibit colon cancer cell proliferation, invasion and migration. We identified MEMO1 as a novel potential target gene of miR-219a-1. Luciferase assays showed that miR-219a-1 could directly bind to 3′-UTR of MEMO1 . Overexpression of miR-219a-1 in colon cancer cells could inhibit the expression of MEMO1 . Furthermore, MEMO1 was upregulated in patients with colon cancer, which was inversely correlated with miR-219a-1 levels. In conclusion, our study revealed that miR-219a-1 exerts anti-tumor effects and regulates colon cancer cell proliferation, invasion and migration by targeting MEMO1 , suggesting that miR-219a-1 could act as a therapeutic target in colon cancer.

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