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miR-219a-1 inhibits colon cancer cells proliferation and invasion by targeting MEMO1

Authors
  • Xu, Keqing1
  • Shi, Jie
  • Mo, Dongping2
  • Yang, Yanhua1
  • Fu, Qiang
  • Luo, Ying3
  • 1 Department of Comprehensive Medical Laboratory, Changzhou No. 7 People’s Hospital, P. R. China
  • 2 Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, P. R. China
  • 3 Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai
Type
Published Article
Journal
Cancer Biology & Therapy
Publisher
Landes Bioscience
Publication Date
Nov 20, 2020
Volume
21
Issue
12
Pages
1163–1170
Identifiers
DOI: 10.1080/15384047.2020.1843897
PMID: 33218285
PMCID: PMC7722797
Source
PubMed Central
Keywords
License
Green

Abstract

Colon cancer is the third most common cancer worldwide. Many miRNAs have been reported to be involved in colon cancer progression. However, there are only a few studies on the role of miR-219a-1 in colon cancer, and the molecular mechanisms involved remain unclear. The aim of this study was to investigate the miR-219a-1 level in patients with colon cancer and to explore both the effects and regulatory mechanisms of miR-219a-1 in the malignancy of colon cancer cells. Real-time PCR and western blot analysis were used to analyze the expression levels of miR-219a-1 and mediator of ErbB2-driven cell motility 1. Cell Counting Kit-8, transwell and wound-healing assays were performed to investigate the malignant ability of colon cancer cells. A luciferase assay was performed to explore whether miR-219a-1 could directly bind to 3ʹ-UTR region of MEMO1 . miR-219a-1 was found to be downregulated in colon cancer cell lines and in patients with colon cancer. Additionally, miR-219a-1 could inhibit colon cancer cell proliferation, invasion and migration. We identified MEMO1 as a novel potential target gene of miR-219a-1. Luciferase assays showed that miR-219a-1 could directly bind to 3′-UTR of MEMO1 . Overexpression of miR-219a-1 in colon cancer cells could inhibit the expression of MEMO1 . Furthermore, MEMO1 was upregulated in patients with colon cancer, which was inversely correlated with miR-219a-1 levels. In conclusion, our study revealed that miR-219a-1 exerts anti-tumor effects and regulates colon cancer cell proliferation, invasion and migration by targeting MEMO1 , suggesting that miR-219a-1 could act as a therapeutic target in colon cancer.

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