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miR-214 represses mitofusin-2 to promote renal tubular apoptosis in ischemic acute kidney injury.

Authors
  • Yan, Yu1, 2, 3
  • Ma, Zhengwei2, 3
  • Zhu, Jiefu1
  • Zeng, Mengru1
  • Liu, Hong1
  • Dong, Zheng1, 2, 3
  • 1 Department of Nephrology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China. , (China)
  • 2 Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, Augusta, Georgia. , (Georgia)
  • 3 Charlie Norwood Veterans Affairs Medical Center, Augusta, Georgia. , (Georgia)
Type
Published Article
Journal
AJP Renal Physiology
Publisher
American Physiological Society
Publication Date
Apr 01, 2020
Volume
318
Issue
4
Identifiers
DOI: 10.1152/ajprenal.00567.2019
PMID: 32003595
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Disruption of mitochondrial dynamics is an important pathogenic event in both acute and chronic kidney diseases, but the underlying mechanism remains poorly understood. Here, we report the regulation of mitofusin-2 (Mfn2; a key mitochondrial fusion protein) by microRNA-214 (miR-214) in renal ischemia-reperfusion that contributes to mitochondrial fragmentation, renal tubular cell death, and ischemic acute kidney injury (AKI). miR-214 was induced, whereas Mfn2 expression was decreased, in mouse ischemic AKI and cultured rat kidney proximal tubular cells (RPTCs) following ATP depletion treatment. Overexpression of miR-214 decreased Mfn2. Conversely, inhibition of miR-214 with anti-miR-214 prevented Mfn2 downregulation in RPTCs following ATP depletion. Anti-miR-214 further ameliorated mitochondrial fragmentation and apoptosis, whereas overexpression of miR-214 increased apoptosis, in ATP-depleted RPTCs. To test regulation in vivo, we established a mouse model with miR-214 specifically deleted from kidney proximal tubular cells (PT-miR-214-/-). Compared with wild-type mice, PT-miR-214-/- mice had less severe tissue damage, fewer apoptotic cells, and better renal function after ischemic AKI. miR-214 induction in ischemic AKI was suppressed in PT-miR-214-/- mice, accompanied by partial preservation of Mfn2 in kidneys. These results unveil the miR-214/Mfn2 axis that contributes to the disruption of mitochondrial dynamics and tubular cell death in ischemic AKI, offering new therapeutic targets.

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