Affordable Access

deepdyve-link
Publisher Website

Minority report: small-scale metagenomic analysis of the non-bacterial kitchen sponge microbiota

Authors
  • Brandau, Lena
  • Jacksch, Susanne
  • Weis, Severin
  • Schnell, Sylvia
  • Egert, Markus
Type
Published Article
Journal
Archives of Microbiology
Publisher
Springer Berlin Heidelberg
Publication Date
Jun 04, 2022
Volume
204
Issue
7
Identifiers
DOI: 10.1007/s00203-022-02969-9
PMID: 35661258
PMCID: PMC9167186
Source
PubMed Central
Keywords
Disciplines
  • Original Paper
License
Unknown

Abstract

Kitchen sponges are particularly well known to harbor a high number and diversity of bacteria, including pathogens. Viruses, archaea, and eukaryotes in kitchen sponges, however, have not been examined in detail so far. To increase knowledge on the non-bacterial kitchen sponge microbiota and its potential hygienic relevance, we investigated five used kitchen sponges by means of metagenomic shot-gun sequencing. Viral particles were sought to be enriched by a filter step during DNA extraction from the sponges. Data analysis revealed that ~ 2% of the sequences could be assigned to non-bacterial taxa. Each sponge harbored different virus (phage) species, while the present archaea were predominantly affiliated with halophilic taxa. Among the eukaryotic taxa, besides harmless algae, or amoebas, mainly DNA from food-left-overs was found. The presented work offers new insights into the complex microbiota of used kitchen sponges and contributes to a better understanding of their hygienic relevance. Supplementary Information The online version contains supplementary material available at 10.1007/s00203-022-02969-9.

Report this publication

Statistics

Seen <100 times