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Millimeter scale separation of DNA with a replaceable polymer matrix.

Authors
  • Manage, Dammika P
  • Elliott, Duncan G
  • Backhouse, Christopher J
Type
Published Article
Journal
Electrophoresis
Publisher
Wiley (John Wiley & Sons)
Publication Date
Nov 01, 2012
Volume
33
Issue
21
Pages
3213–3221
Identifiers
DOI: 10.1002/elps.201200188
PMID: 23027089
Source
Medline
License
Unknown

Abstract

Electrophoresis is a powerful method that has seen a wide range of applications, often in automated genetic diagnostic instruments that require the use of a replaceable sieving matrix. The power and simplicity of electrophoresis as an analysis technique would be ideal for highly integrated and low-cost analysis systems if the method could be implemented in microfluidics on the scale of several mm. We demonstrate the electrophoretic analysis of DNA with separation lengths as small as 2 mm and with a resolution adequate for the analysis of PCR products, i.e. resolutions of 10-20 base pairs. Such small-scale separations enable analysis systems consisting of microfluidics and microelectronics integrated into a single inexpensive package, thereby overcoming a key challenge facing the development of the lab on chip technologies.

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