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Milestones in electron crystallography

Authors
  • Renault, Ludovic1
  • Chou, Hui -Ting1
  • Chiu, Po -Lin1
  • Hill, Rena M.1
  • Zeng, Xiangyan1
  • Gipson, Bryant1
  • Zhang, Zi Yan1
  • Cheng, Anchi2
  • Unger, Vinzenz3
  • Stahlberg, Henning1
  • 1 University of California at Davis, Molecular and Cellular Biology, College of Biological Sciences, Briggs Hall, 1 Shields Ave., Davis, CA, 95616, USA , Davis (United States)
  • 2 The Scripps Research Institute, Department of Cell Biology, 1055 N. Torrey Pines Road, La Jolla, CA, 02037, USA , La Jolla (United States)
  • 3 Yale University, Department of Molecular Biophysics and Biochemistry, New Haven, CT, 065200-8024, USA , New Haven (United States)
Type
Published Article
Journal
Journal of Computer-Aided Molecular Design
Publisher
Springer-Verlag
Publication Date
Aug 01, 2006
Volume
20
Issue
7-8
Pages
519–527
Identifiers
DOI: 10.1007/s10822-006-9075-x
Source
Springer Nature
Keywords
License
Yellow

Abstract

Electron crystallography determines the structure of membrane embedded proteins in the two-dimensionally crystallized state by cryo-transmission electron microscopy imaging and computer structure reconstruction. Milestones on the path to the structure are high-level expression, purification of functional protein, reconstitution into two-dimensional lipid membrane crystals, high-resolution imaging, and structure determination by computer image processing. Here we review the current state of these methods. We also created an Internet information exchange platform for electron crystallography, where guidelines for imaging and data processing method are maintained. The server (http://2dx.org) provides the electron crystallography community with a central information exchange platform, which is structured in blog and Wiki form, allowing visitors to add comments or discussions. It currently offers a detailed step-by-step introduction to image processing with the MRC software program. The server is also a repository for the 2dx software package, a user-friendly image processing system for 2D membrane protein crystals.

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