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Mikrocytos mytilicoli n.sp. (Cercozoa, Mikrocytida, Mikrocytiidae) infecting the copepod Mytilicola intestinalis (Arthropoda, Cyclopoida, Mytilicolidae), a symbiont of Mytilus galloprovincialis in Galicia (NW Spain).

Authors
  • Darriba, Susana1
  • Lee, Ren-Shiang2
  • López, Carmen3
  • 1 Instituto Tecnolóxico para o Control do Medio Mariño de Galicia (INTECMAR), Consellería do Mar, Xunta de Galicia, Peirao de Vilaxoán s/n, 36611 Vilagarcía de, Arousa, Pontevedra, Spain. , (Spain)
  • 2 Departamento I+D+i, Consello Regulador do Mexillón de Galicia, Avda da Mariña, 25, 36600 Vilagarcía de Arousa, Pontevedra, Spain. , (Spain)
  • 3 Centro de Investigacións Mariñas (CIMA), Consellería do Mar, Xunta de Galícia, Pedras de Corón s/n, 36620 Vilanova de Arousa, Pontevedra, Spain. Electronic address: [email protected] , (Spain)
Type
Published Article
Journal
Journal of Invertebrate Pathology
Publisher
Elsevier
Publication Date
Sep 03, 2020
Volume
176
Pages
107460–107460
Identifiers
DOI: 10.1016/j.jip.2020.107460
PMID: 32891682
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

During a histopathological survey of Mytilus galloprovincialis in Galicia (NW Spain), microcells were observed infecting several organs of the symbiont copepod Mytilicola intestinalis. Positive results of PCR assay with specific primers for genus Mikrocytos and a clear signal of in situ hybridization with MACKINI-1 digoxigenin- labelled DNA probe (DIG-ISH) indicated a protozoan parasite of Mikrocytos genus. The ultrastructural study revealed intra and extracellular locations, polymorphic nuclei, intracellular round vesicles in the cytoplasm and absence of mitochondria. The present paper reports the characterization of the Mikrocytos sp. infecting M. intestinalis and proposes a novel species in the genus: Mikrocytos mytilicoli n. sp. A sequence of 18S-28S rDNA was obtained with 95.6% maximum identity (query cover 100%) with Mikrocytos mackini. Phylogenetic analysis showed that M. mytilicoli n. sp. and M. mackini share a common ancestor. However, comparison of the ITS1 rDNA region showed low similarity (75.8%) with M. mackini, which, combined with differences in ultrastructural details, host and geographic location, support the designation of a new species. This is the first description of a microcytid parasite of the genus Mikrocytos from a non-bivalve host. Copyright © 2020 Elsevier Inc. All rights reserved.

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