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Microwave fixation of the lung.

Authors
  • Turner, C R
  • Zuczek, S
  • Knudsen, D J
  • Wheeldon, E B
Type
Published Article
Journal
Stain technology
Publication Date
Jan 01, 1990
Volume
65
Issue
2
Pages
95–101
Identifiers
PMID: 2360216
Source
Medline
License
Unknown

Abstract

A technique for microwave fixation of inflated rat lung is described. Conventional intratracheal fixation with instillation of fixative into the airways at a constant pressure results in pressure artifacts as well as flushing and disruption of cells and exudates. Microwave fixation fixes these elements in situ without disruption and thus is valuable when evaluating the distribution of inflammatory infiltrates. Exudative pneumonitis was produced in the rat using intratracheal instillations of either endotoxin or silica and comparisons were made between histologic sections fixed using either standard formalin fixation or microwave fixation.

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