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MicroRNA-466a-3p attenuates allergic nasal inflammation in mice by targeting GATA3.

Authors
  • Chen, Z1
  • Deng, Y2
  • Li, F2, 3
  • Xiao, B2, 3
  • Zhou, X1
  • Tao, Z2, 3
  • 1 Department of Otolaryngology Head and Neck Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China. , (China)
  • 2 Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, China. , (China)
  • 3 Research Institute of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, China. , (China)
Type
Published Article
Journal
Clinical & Experimental Immunology
Publisher
Wiley (Blackwell Publishing)
Publication Date
Sep 01, 2019
Volume
197
Issue
3
Pages
366–375
Identifiers
DOI: 10.1111/cei.13312
PMID: 31081939
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Allergic rhinitis is thought to be an allergic disease associated with immunoglobulin (Ig)E-mediated immune response, characterized by increased T helper type 2 (Th2) cytokine production, elevated eosinophil levels in the nasal mucosa and induced nasal secretions. MicroRNA (miRNA) microarray data revealed that the expression level of miR-466a-3p was significantly decreased. Notably, GATA binding protein (GATA-3) was identified as one of its target genes through miRNA target prediction web tools. The expression levels of miR-466a-3p were altered by mimics and lentivirus both in vivo and in vitro, similar to those of GATA-3. Furthermore, the symptoms and histology of allergic rhinitis as well as the levels of serum IgE and interleukin (IL)-4 were examined in different groups of mice. Interestingly, the results for lentiviral miR-466a-3p-treated allergic rhinitis mice were relatively similar to normal mice, compared to allergic rhinitis mice without treatment. Also, miR-466a-3p negatively regulated GATA-3 expression in allergic rhinitis mice, indicating the participant of Th2-cell responses in allergic rhinitis. Taken together, our findings highlight a new perspective on the role of miR-466a-3p in allergic rhinitis. In addition, this study provides a theoretical framework and experimental reference for future research targeting microRNAs as therapeutic targets and diagnostic biomarkers of allergic rhinitis. © 2019 British Society for Immunology.

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