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Microinjection of the ras oncogene protein into nonestablished rat embryo fibroblasts.

Authors
Type
Published Article
Journal
Cancer research
Publication Date
Volume
46
Issue
12 Pt 1
Pages
6427–6432
Identifiers
PMID: 3536079
Source
Medline

Abstract

Early (twice) and late (eighth) passage nonestablished rat embryo fibroblasts exhibit dramatically different responses to microinjection of the oncogenic form (T24) of the ras protein. Late passage cells respond like established cultures in that they both synthesize DNA and divide in response to the oncogenic protein. However, early passage cells are shown to be much less responsive to the introduction of this protein in terms of the threshold concentration required to elicit a mitogenic response. Furthermore, conditioned medium from late passage cells can both potentiate the effect of ras in early passage cells and stimulate colony formation in soft agar. In addition, we show that human cells can respond to microinjection of the ras oncogene protein by synthesizing DNA.

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