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Microfluidic quantification of multiple enteric and opportunistic bacterial pathogens in roof-harvested rainwater tank samples

Authors
  • Ahmed, Warish1
  • Zhang, Qian2
  • Ishii, Satoshi2
  • Hamilton, Kerry3
  • Haas, Charles3
  • 1 CSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, Dutton Park, Queensland, 4102, Australia , Dutton Park (Australia)
  • 2 University of Minnesota, BioTechnology Institute, Saint Paul, MN, 55108, USA , Saint Paul (United States)
  • 3 Drexel University, 3141 Chestnut Street, Philadelphia, PA, 19104, USA , Philadelphia (United States)
Type
Published Article
Journal
Environmental Monitoring and Assessment
Publisher
Springer-Verlag
Publication Date
Jan 30, 2018
Volume
190
Issue
2
Identifiers
DOI: 10.1007/s10661-018-6482-0
Source
Springer Nature
Keywords
License
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Abstract

Potable and non-potable uses of roof-harvested rainwater (RHRW) are increasing due to water shortages. To protect human health risks, it is important to identify and quantify disease-causing pathogens in RHRW so that appropriate treatment options can be implemented. We used a microfluidic quantitative PCR (MFQPCR) system for the quantitative detection of a wide array of fecal indicator bacteria (FIB) and pathogens in RHRW tank samples along with culturable FIB and conventional qPCR analysis of selected pathogens. Among the nine pathogenic bacteria and their associated genes tested with the MFQPCR, 4.86 and 2.77% samples were positive for Legionella pneumophila and Shigella spp., respectively. The remaining seven pathogens were absent. MFQPCR and conventional qPCR results showed good agreement. Therefore, direct pathogen quantification by MFQPCR systems may be advantageous for circumstances where a thorough microbial analysis is required to assess the public health risks from multiple pathogens that occur simultaneously in the target water source.

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