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Microfluidic isolation of leukocytes from whole blood for phenotype and gene expression analysis.

Authors
  • Sethu, Palaniappan
  • Moldawer, Lyle L
  • Mindrinos, Michael N
  • Scumpia, Philip O
  • Tannahill, Cynthia L
  • Wilhelmy, Julie
  • Efron, Philip A
  • Brownstein, Bernard H
  • Tompkins, Ronald G
  • Toner, Mehmet
Type
Published Article
Journal
Analytical chemistry
Publication Date
Aug 01, 2006
Volume
78
Issue
15
Pages
5453–5461
Identifiers
PMID: 16878882
Source
Medline
License
Unknown

Abstract

Technologies that enable the isolation of cell subtypes from small samples of complex populations will greatly facilitate the implementation of proteomics and genomics to human diseases. Transcriptome analysis of blood requires the depletion of contaminating erythrocytes. We report an automated microfluidic device to rapidly deplete erythrocytes from whole blood via deionized water lysis and to collect enriched leukocytes for phenotype and genomic analyses. Starting with blood from healthy subjects, we demonstrate the utility of this microfluidic cassette and lysis protocol to prepare unstimulated leukocytes, and leukocytes stimulated ex vivo with Staphylococcal enterotoxin B, which mimics some of the cellular effects seen in patients with severe bacterial infections. Microarrays are used to assess the global gene expression response to enterotoxin B. The results demonstrate that this system can isolate unactivated leukocytes from small blood samples without any significant loss, which permits more information to be obtained from subsequent analysis, and will be readily applicable to clinical settings.

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