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Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography.

Authors
  • K, Liu
  • Ej, Lepin
  • Mw, Wang
  • F, Guo
  • Wy, Lin
  • Yc, Chen
  • Sj, Sirk
  • S, Olma
  • Me, Phelps
  • Xz, Zhao
  • Hr, Tseng
  • R, Michael Van Dam
  • Am, Wu
  • Ck, Shen
Type
Published Article
Journal
Molecular Imaging
Publisher
SAGE Publications
Volume
10
Issue
3
Source
van Dam Lab
License
Unknown

Abstract

Methods for tagging biomolecules with fluorine 18 as immuno-positron emission tomography (immunoPET) tracers require tedious optimization of radiolabeling conditions and can consume large amounts of scarce biomolecules. We describe an improved method using a digital microfluidic droplet generation (DMDG) chip, which provides computer-controlled metering and mixing of 18F tag, biomolecule, and buffer in defined ratios, allowing rapid scouting of reaction conditions in nanoliter volumes. The identified optimized conditions were then translated to bench-scale 18F labeling of a cancer-specific engineered antibody fragments, enabling microPET imaging of tumors in xenografted mice at 0.5 to 4 hours postinjection.

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