Affordable Access

deepdyve-link
Publisher Website

Microbiome diversity in the bronchial tracts of patients with chronic obstructive pulmonary disease.

Authors
  • Cabrera-Rubio, Raúl1
  • Garcia-Núñez, Marian
  • Setó, Laia
  • Antó, Josep M
  • Moya, Andrés
  • Monsó, Eduard
  • Mira, Alex
  • 1 Department of Genomics and Health, Center for Public Health Research, Valencia, Spain. , (Spain)
Type
Published Article
Journal
Journal of Clinical Microbiology
Publisher
American Society for Microbiology
Publication Date
Nov 01, 2012
Volume
50
Issue
11
Pages
3562–3568
Identifiers
DOI: 10.1128/JCM.00767-12
PMID: 22915614
Source
Medline
License
Unknown

Abstract

Culture of bacteria from bronchial secretions in respiratory patients has low sensitivity and does not allow for complete assessment of microbial diversity across different bronchial compartments. In addition, a significant number of clinical studies are based on sputum samples, and it is not known to what extent they describe the real diversity of the mucosa. In order to identify previously unrecognized lower airway bacteria and to investigate the complexity and distribution of microbiota in patients with chronic obstructive pulmonary disease (COPD), we performed PCR amplification and pyrosequencing of the 16S rRNA gene in patients not showing signs or symptoms of infection. Four types of respiratory samples (sputum, bronchial aspirate, bronchoalveolar lavage, and bronchial mucosa) were taken from each individual, obtaining on average >1,000 16S rRNA sequences per sample. The total number of genera per patient was >100, showing a high diversity, with Streptococcus, Prevotella, Moraxella, Haemophilus, Acinetobacter, Fusobacterium, and Neisseria being the most commonly identified. Sputum samples showed significantly lower diversity than the other three sample types. Lower-bronchial-tree samples, i.e., bronchoalveolar lavage and bronchial mucosa, showed a very similar bacterial compositions in contrast to sputum and bronchial aspirate samples. Thus, sputum and bronchial aspirate samples are upper bronchial tree samples that are not representative of the lower bronchial mucosa flora, and bronchoalveolar lavage samples showed the results closest to those for the bronchial mucosa. Our data confirm that the bronchial tree is not sterile in COPD patients and support the existence a different microbiota in the upper and lower compartments.

Report this publication

Statistics

Seen <100 times