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Microbiological assay for vitamin B12 performed in 96-well microtitre plates.

Authors
  • B P Kelleher
  • S D Broin
Publication Date
Jul 01, 1991
Source
PMC
Keywords
Disciplines
  • Biology
  • Medicine
License
Unknown

Abstract

A simplified microbiological assay for vitamin B12 estimation, completed on microtitre plates using a colistin sulphate resistant strain of Lactobacillus leichmannii (NCIB 12519), and cryopreserved cultures is described. The new assay correlated well with a more conventional "tube" assay and was not influenced by the presence of antibiotics in serum. Evaluation of assay performance showed excellent interassay and intra-assay precision with quantitative recovery of added cyanocobalamin over a wide range of additions (94.9%-102.1%). The advantages of short incubation time, easy reading, and minimal reagent costs make this assay an attractive option in the routine clinical laboratory and in research.

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