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Microbial diversity of acidic hot spring (kawah hujan B) in geothermal field of kamojang area, west java-indonesia.

Authors
  • Aditiawati, Pingkan1
  • Yohandini, Heni
  • Madayanti, Fida
  • Akhmaloka
  • 1 School of Life Science and Technology, Institut Teknologi Bandung, Jl Ganesha 10, Bandung, Indonesia. , (Indonesia)
Type
Published Article
Journal
The Open Microbiology Journal
Publisher
Bentham Science
Publication Date
2009
Volume
3
Pages
58–66
Identifiers
DOI: 10.2174/1874285800903010058
PMID: 19440252
Source
Medline
Keywords
License
Unknown

Abstract

Microbial communities in an acidic hot spring, namely Kawah Hujan B, at Kamojang geothermal field, West Java-Indonesia was examined using culture dependent and culture independent strategies. Chemical analysis of the hot spring water showed a characteristic of acidic-sulfate geothermal activity that contained high sulfate concentrations and low pH values (pH 1.8 to 1.9). Microbial community present in the spring was characterized by 16S rRNA gene combined with denaturing gradient gel electrophoresis (DGGE) analysis. The majority of the sequences recovered from culture-independent method were closely related to Crenarchaeota and Proteobacteria phyla. However, detail comparison among the member of Crenarchaeota showing some sequences variation compared to that the published data especially on the hypervariable and variable regions. In addition, the sequences did not belong to certain genus. Meanwhile, the 16S Rdna sequences from culture-dependent samples revealed mostly close to Firmicute and gamma Proteobacteria.

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