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Microautoradiography of Water-Soluble Compounds in Plant Tissue after Freeze-Drying and Pressure Infiltration with Epoxy Resin

Authors
  • Thomas C. Vogelmann
  • Richard E. Dickson
Publication Date
Aug 01, 1982
Source
PMC
Keywords
License
Unknown

Abstract

It is difficult to retain and localize radioactive, water-soluble compounds within plant cells. Existing techniques retain water-soluble compounds with varying rates of efficiency and are limited to processing only a few samples at one time. We developed a modified pressure infiltration technique for the preparation of microautoradiographs of 14C-labeled, water-soluble compounds in plant tissue. Samples from cottonwood (Populus deltoides Bartr. ex Marsh.) labeled with 14C were excised, quick frozen in liquid N2, freeze-dried at −50°C, and pressure-infiltrated with epoxy resin without intermediate solvents or prolonged incubation times. The technique facilitates the mass processing of samples for microautoradiography, gives good cellular retention of labeled water-soluble compounds, and is highly reproducible.

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