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Microarray-Based Detection of Allergen-Reactive IgE in Patients with Mastocytosis.

Authors
  • Smiljkovic, Dubravka1
  • Kiss, Renata2
  • Lupinek, Christian2
  • Hoermann, Gregor3
  • Greiner, Georg4
  • Witzeneder, Nadine5
  • Krajnik, Gerhard6
  • Trautinger, Franz7
  • Vrtala, Susanne2
  • Mittermann, Irene2
  • Kundi, Michael8
  • Jilma, Bernd9
  • Valenta, Rudolf10
  • Sperr, Wolfgang R11
  • Valent, Peter12
  • 1 Department of Internal Medicine I, Division of Hematology & Hemostaseology, Medical University of Vienna, Vienna, Austria. , (Austria)
  • 2 Department of Pathophysiology and Allergy Research, Division of Immunopathology, Center for Pathophysiology, Immunology and Infectiology, Medical University of Vienna, Vienna, Austria. , (Austria)
  • 3 Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria; Ludwig Boltzmann Institute for Hematology and Oncology, Medical University of Vienna, Vienna, Austria; Central Institute of Medical and Chemical Laboratory Diagnostics, University Hospital Innsbruck, Innsbruck, Austria. , (Austria)
  • 4 Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria; Ludwig Boltzmann Institute for Hematology and Oncology, Medical University of Vienna, Vienna, Austria. , (Austria)
  • 5 Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria. , (Austria)
  • 6 Department of Internal Medicine I, University Hospital St. Poelten, St. Poelten, Austria. , (Austria)
  • 7 Department of Dermatology, University Hospital St. Poelten, St. Poelten, Austria. , (Austria)
  • 8 Institute of Environmental Health of the Medical University of Vienna, Vienna, Austria. , (Austria)
  • 9 Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria. , (Austria)
  • 10 Department of Pathophysiology and Allergy Research, Division of Immunopathology, Center for Pathophysiology, Immunology and Infectiology, Medical University of Vienna, Vienna, Austria; NRC Institute of Immunology FMBA of Russia, Moscow, Russia; Laboratory for Immunopathology, Department of Clinical Immunology and Allergy, Sechenov First Moscow State Medical University, Moscow, Russia; Karl Landsteiner University of Health Sciences, Krems, Austria. , (Austria)
  • 11 Department of Internal Medicine I, Division of Hematology & Hemostaseology, Medical University of Vienna, Vienna, Austria; Ludwig Boltzmann Institute for Hematology and Oncology, Medical University of Vienna, Vienna, Austria. Electronic address: [email protected] , (Austria)
  • 12 Department of Internal Medicine I, Division of Hematology & Hemostaseology, Medical University of Vienna, Vienna, Austria; Ludwig Boltzmann Institute for Hematology and Oncology, Medical University of Vienna, Vienna, Austria. Electronic address: [email protected] , (Austria)
Type
Published Article
Journal
The journal of allergy and clinical immunology. In practice
Publication Date
Sep 01, 2020
Volume
8
Issue
8
Identifiers
DOI: 10.1016/j.jaip.2020.04.030
PMID: 32348913
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Because of a high risk to develop fatal anaphylaxis, early detection of immunoglobulin E (IgE)-dependent allergy is of particular importance in patients with mastocytosis. We examined whether microarray-based screening for allergen-reactive IgE (allergen-chip) is a sensitive and robust approach to detect specific IgE in patients with mastocytosis. Sera for 42 patients were analyzed, including 4 with cutaneous mastocytosis, 2 with mastocytosis in the skin, and 36 with systemic mastocytosis. In addition, sera from an age- and sex-matched control cohort (n = 42) were analyzed. In 15 of 42 patients with mastocytosis (35.7%), specific IgE was detected by allergen-chip profiling. Ves v 5 and Bet v 1 were the most frequently detected allergens (Ves v 5: 16.7% of patients; Bet v 1: 11.9% of patients). Allergen reactivity was confirmed by demonstrating upregulation of CD203c on blood basophils upon exposure to the respective allergen(s) in these patients. Specific IgE was identified by chip studies in 11 of 26 patients with mastocytosis with mediator-related symptoms (42.3%) and in 4 of 14 patients with mastocytosis without symptoms (28.6%). In the cohort with known allergy, 9 of 9 patients (100%) had a positive allergen-chip result. In patients with mastocytosis without a known allergy (n = 31), the chip identified 6 positive cases (19.5%). The prevalence of chip-positive patients was slightly lower in the mastocytosis group (35.7%) compared with age- and sex-matched controls (40.5%). Although specific IgE may not be detectable in all sensitized patients with mastocytosis, allergy chip-profiling is a reliable screening approach for the identification of patients with mastocytosis suffering from IgE-dependent allergies. Copyright © 2020 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

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