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Microarray analysis of acaricide-inducible gene expression in the southern cattle tick, Rhipicephalus (Boophilus) microplus.

Authors
  • Saldivar, L1
  • Guerrero, F D
  • Miller, R J
  • Bendele, K G
  • Gondro, C
  • Brayton, K A
  • 1 USDA-ARS Knipling-Bushland US Livestock Insects Research Laboratory, Kerrville, TX 78028, USA.
Type
Published Article
Journal
Insect Molecular Biology
Publisher
Wiley (Blackwell Publishing)
Publication Date
December 2008
Volume
17
Issue
6
Pages
597–606
Identifiers
DOI: 10.1111/j.1365-2583.2008.00831.x
PMID: 18834453
Source
Medline
License
Unknown

Abstract

Acaricide-inducible differential gene expression was studied in larvae of Rhipicephalus (Boophilus) microplus using a microarray-based approach. The acaricides used were: coumaphos, permethrin, ivermectin, and amitraz. The microarrays contained over 13 000 probes, having been derived from a previously described R. microplus gene index (BmiGI Version 2; Wang et al., 2007). Relative quantitative reverse transcriptase-PCR, real time PCR, and serial analysis of gene expression data was used to verify microarray data. Among the differentially expressed genes with informative annotation were legumain, glutathione S-transferase, and a putative salivary gland-associated protein.

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