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Micro-injection of Morpholino oligonucleotides for depleting Securin in mouse oocytes.

Authors
  • Marangos, Petros
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2013
Volume
957
Pages
153–162
Identifiers
DOI: 10.1007/978-1-62703-191-2_10
PMID: 23138950
Source
Medline
License
Unknown

Abstract

Gene silencing techniques have brought new insights into mammalian oocyte and embryo development. More specifically, the use of Morpholino oligonucleotides which sterically inhibit translation from target mRNAs thereby compromising gene function, allowed the identification of important oocyte regulators and especially factors involved in meiotic cell cycle control. Here we describe the method of application of Morpholino oligonucleotides in mouse oocyte research.

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